Chromatography data
We are about to start the lab in fourth period. Here is the actual data I collected in 3rd period (AP Biology).
Figure out Rf values:
Data (distance in mm):
Solvent traveled: 111mm
carotene: 109mm
xanthophyll: 43mm
chlorophyll a: 21mm
chlorophyll b: 11mm
To calculate the value: the distance pigment traveled divided by the distance the solvent traveled.
By Monday (the day after break) turn in the following:
Your Rf values (or calculate using mine)
A paragraph (5+ sentences) on how chromatography is used by actual people in the real world (scientists, CSI's, etc.)
posted by jm at 1:39 PM
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9 Comments:
Also by Monday:
Read chapter 5-3, use the handout from Friday as your guide. Don't forget to check the web site for animations, or if you find some let me know and I'll add them.
Review all of chapter 5. Plus the links from the web site.
Turn in the Rf values and the paragraph on why we do chromatography (preferred typed, but not copied and pasted).
Take a few days off and relax.
12:32 AM
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7:40 PM
1. Don't post anonymously; sign your name even if you post anonymously
2. I gave instructions in the main post:
To calculate the value: the distance pigment traveled divided by the distance the solvent traveled.
Rf = pigment/solvent
so if the solvent traveled 2 miles and the pigment (xanthophyll, chlorophyll, etc) traveled 1 mile then the value = 1/2.
Does the Rf value change if the solvent changes? Post that to the blog. We used ether/acetone, so I bet there are published values for this solvent.
8:18 PM
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2:24 PM
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8:24 PM
Don't post anonymously. Sign your name if you did not log in to blogger.
As for the lab: 1st period did it Friday, 4th did it on Wednesday. Instructions are in the post above.
3:53 AM
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8:28 PM
I thought the electrons travelled through the e-?
Venezuela
6:13 PM
e- = electrons.
In cellular respiration they travel from NAD and FADH2 through the electron transport chain to oxygen, where hydrogen ions are added to make water.
9:59 PM
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